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KMID : 1134820110400111507
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 11 p.1507 ~ p.1511
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Anti-Inflammatory Effect of Erigeron annuus L. Flower Extract through Heme Oxygenase-1 Induction in RAW264.7 Macrophages
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Sung Mi-sun
Kim Young-Wha
Choi Young-Min
Ham Hyeon-Mi
Jeong Heon-Sang
Lee Jun-Soo
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Abstract
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This study investigated the anti-inflammatory effect of Erigeron annuus L. flower (EAF) methanol extract. We examined the involvement of heme oxygenase-1 (HO-1) in the inhibitory activities of EAF methanol extract on nitric oxide (NO)production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Cell viability and NO assays were performed. In addition, inducible nitric oxide synthase (iNOS) and HO-1 expressions were detected by Western blotting and blocking HO-1 activity on NO production. EAF methanol extract (25, 50, 100, 200 μg/mL) significantly inhibited LPS-stimulated NO production (p<0.05; 12.82, 9.61, 6.83, 2.52 μM) in a concentration- dependent manner. EAF methanol extract also reduced the expression of iNOS protein. The EAF methanol extract induced the expression of HO-1 in a dose-dependent manner. Blockage of HO-1 activity by zinc protoporphyrin suppressed EAF methanol extract-induced reductions in the production of NO. The present results suggest that EAF methanol extract has a potent anti-inflammatory effect in RAW264.7 macrophages through the induction of HO-1.
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KEYWORD
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Erigeron annuus, anti-inflammation, nitric oxide, inducible nitric oxide synthase, heme oxygenase-1
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